Keep out of reach of children and unauthorized persons. Avoid contact with skin, eyes, and mucous membranes. Store in a cool, dry location away from sunlight.
The rate of ion depletion due to algal uptake is driven by various chemical and physical factors which are unique to each independently-operating recirculating system, and which are constantly changing as algal biomass increases and decreases.
Monitoring (daily) the system's concentrations of elements provided by this formulation, as well as the concentration of nitrate, and referencing apparent changes in algal biomass will enable User to adjust dosing of this formula to suit system requirements, improving overall effectiveness and resulting in less wasted solution.
Users unable to perform analyses for elements provided by this formula are recommended to monitor nitrate concentration and apparent changes in algal biomass, adjusting dosage to suit system requirements. This visual approach to dosing is fastest, and for many systems will be adequate.
Recommended is diluting an appropriate volume of this solution into a larger volume of purified water (with a purity of at least 98%), and applying this diluted solution to the system with an automated dosing system throughout the photoperiod. Dosing frequency is directly proportional to stability of ionic concentrations
and, resultantly, continuous algal biomass growth. A 1 - 10 s drip interval during the photoperiod provides adequate ionic stability, and is recommended.
If no automated dosing system is available, then apply solution manually each day. Dosing frequency is directly proportional to stability of ionic concentrations and, resultantly, continuous algal biomass growth. Dosage volume per 24 h is divided by the dosing frequency*.
It is not recommended that this formulation be dosed fewer than four times weekly.
Reference Standards: 1 mL per 250 gal. (946.25 L). If using glass dropper supplied with 30- and 60-mL package, then 1 mL is equivalent to approximately 20 drops. 1 drop per 12.5 gal. (47.3125 L). For laboratory applications, dilute 1 mL Isol8 ATS into 99 mL of distilled or deionized water, and apply at 1 mL per 2.5 gal as necessary.
Initial application: Dose equivalent of 1 mL per 500 gal. (1892.5 L) every 24 h, during photoperiod**. Monitor nitrate concentration at beginning, middle, and end of photoperiod or, if photoperiod length is 24 h, at three regular times each day over a 10 d period. A decrease in peak nitrate concentration recorded at the same reference times indicates increased nitrate uptake and depletion of the elements provided by this formulation.
Increase dose by 1 - 5% every 120 h until reference nitrate concentration ceases to decrease. Objective is for nitrate concentration to remain within 5% of lowest recorded value. Adjust dosing volume to maintain that concentration, +/-5%.
*Example: If dosage volume per 24 h = 10 mL and dosing frequency = 4, then dose 2.5 mL per event.
**Example: If photoperiod length is 24 h and dosing volume is 10 mL, then meter dosing of 10 mL throughout the entire 24 h period. If photoperiod is 8 h and dosing volume is 10 mL, then meter dosing of 10 mL throughout the 8 h period.
Algae, including those commonly employed in turf scrubbers, utilize specific minor and trace elements during the course of various cellular functions, including, but not limited to: cell division; nitrogen uptake (nitrogen assimilation, nitrate reduction); CO2 fixation; phosphorus acquisition; photosynthesis; production of pigments and vitamins; DNA transcription. The rate of algae-driven element depletion within a recirculating system is mediated by photoperiod length and rate of nutrient input, among other chemical and physical factors. Failure to maintain adequate availability of these critical elements suppresses the rate of nutrient uptake, feasibly resulting in nutrient accumulation within the system and, potentially, cell death within the algal turf population. Many complex reactions taking place within plant and algal cells require the presence of multiple elements to achieve completion. Absence of any one of the critical elements therefore inhibits productivity.
Elements provided by this formulation: have been long-established by researchers as being critical to plant and algae growth; are present in appropriate ratios; dosed as directed, will not cause growth suppression as a result of accumulation. This formulation does not propose to act as a complete growth medium for diatom cultures, and therefore omits silicon as a component.
Supplementation of minor and/or trace elements known to suppress the growth of plants and algae is contraindicated in systems supported by turf scrubbers. Evidence suggests that presence of Lanthanides, in addition to specific heavy metals (e.g. Hg, Pb, As, Cd, Cr), inhibits growth of algal cultures. These elements are omitted from this formulation.
Minor and Trace Element Solution for Turf Scrubber-dependent Recirculating Saltwater / Brackish Systems